Ercole I. (1431-1505), Duke of Ferrara, Reggio and Modena

Leonora (or Eleonora) of Aragon (1450-1493), daughter of King Ferrante of Naples († 1494)

1. Anna Maria Sforza (1476-1497), daughter of Duke Galeazzo Maria Sforza of Milan († 1476, assassinated); marriage on 22. January 1491
2. Lucrezia Borgia, illegitimate daughter of Pope Alexander VI. († 1503)
3. Laura Eustochia Dianti († 1573)

from his second marriage to Lucrezia Borgia, see: Lucrezia Borgia

from his third marriage (a clandestine, morganatic marriage) to Laura Eustochia Dianti:

1. his son Alfonso, Margrave of Montecchio, born on 10. March 1527, died on 1. November 1587; 1. marriage to Giulia della Rovere (1527-1563), daughter of Francesco Maria I. della Rovere (1490-1538), Duke of Urbino, in 1549 and 2. marriage to Violanta Signa (1546-1609) in 1584; his children by his first marriage were: Alfonsino (1560-1578), Eleonora (1561-1637), and Cesare I. (1562-1628), Duke of Modena and Reggio; his illegitimate children were: Ippolita (1565-1602), and Alessandro (1568-1624), bishop of Reggio and Cardinal
2. his son Alfonsino, born in 1530, died in 1547

• Maike Vogt-Luerssen: Lucrezia Borgia – The Life of a Pope's Daughter in the Renaissance