in the year 1522

18. December 1562

Don Pedro Alvarez of Toledo († 1553), the Spanish Viceroy of Naples from 1532 until 1553

Maria Pimentel y Osorio († 1530), the second Marchioness of Villafranca del Bierzo

Cosimo I. de' Medici (1519-1574), Grand Duke of Tuscany; marriage on 29. March 1539

1. her daughter Maria, born on 3. April 1540, deceased on 19. November 1557
2. her son Francesco I., Grand Duke of Tuscany, born on 25. March 1541, deceased on 20. October 1587
3. her daughter Isabella, born on 31. August 1542, deceased on 16. July 1576 (allegedly strangled by her husband, Paolo Giordano I. Orsini (1541-1585), Duke of Bracciano, though cause of death was more likely a heart attack or stroke); Isabella and Paolo Giordano had two children: Eleonora (1571-1634) and Virginio (1572-1615)
4. her son Giovanni, Cardinal since 1560, born on 28. September 1543, deceased on 20. November 1562
5. her son Antonio, born in July oder August 1544, deceased in 1548
6. her daughter Lucrezia, born on 7. June 1545, deceased on 21. April 1561, married to Alfonso II., Duke of Ferrara
7. her son Pedricco (or Piero), born on 10. August 1546, deceased on 10. June 1547
8. her son Garzia, born on 5. July 1547, deceased on 12. December 1562
9. her son Ferdinand I., Cardinal from 1563 until 1588, Grand Duke of Tuscany since 1588; born on 30. July 1549, deceased on 7. February 1609
10. her daughter Anna, born on 22. February 1553, deceased in 1553
11. her son Pietro, born on 3. June 1554, deceased on 25. April 1604; in April 1571 he was married to his cousin Eleonora of Toledo (1553-1576), whom he allegedly murdered on 7. July 1576